Protocol Additional
Materials PDF
Mouse embryonic fibroblasts PDF
Brl-conditioned medium PDF
Testing FCS batches for culture of embryonic stem cells PDF
ES cell culture PDF
Electroporation PDF
Culturing of selected colonies on 96-well multidish PDF
Subcloning PDF
Chromosome spreads and karyotyping PDF
Preparation of ES cells for blastocyst injection PDF
Crossing scheme chimera => heterozygote => homozygote PDF
Selection for double knock-out ES cells PDF
Introduction of subtle mutations using in-out targeting PDF
Introduction of subtle mutations using cre/Lox PDF
LacZ staining PDF
Differentiation/culture of embryoid bodies PDF
Gpi analysis PDF
Lipofection PDF
Colony assay PDF
ES cell lines-marker genes PDF